M.D., Case Western Reserve University, Medicine, 1990.
The Fredicks laboratory develops and applies molecular diagnostic tests for the detection and identification of microbial pathogens, including those pathogens that are difficult to culture in the laboratory. We employ the polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) to detect and localize bacteria and fungi in human tissues. These molecular approaches are applied to improve the detection of well known pathogens, and to investigate the role of novel or uncultivated microbes in unexplained human diseases (pathogen discovery). The laboratory has developed a suite of PCR assays for the detection of fungal pathogens in immunocompromised patients.
We also study the constituents of the human indigenous microbiota, using cultivation-independent approaches to characterize microbes present on human mucosal and epithelial surfaces. The microbiology of the human vagina is an area of research focus, and we are particularly interested in the role of fastidious bacteria in the syndrome bacterial vaginosis. We also study the role of fastidious vaginal bacteria in other adverse health conditions in women.
Previous research has been performed on the cultivation-resistant pathogens Tropheryma whipplei (bacterial cause of Whipple Disease) and Rhinosporidium seeberi (protistan parasite that causes nasal and ocular tumors in rhinosporidiosis).
American Association for the Advancement of Science
American Society for Microbiology
Infectious Diseases Society of America
Honors and Awards
Physician Scientist Award, National Institutes of Health, Stanford University
Program Commitee Choice Award, Infectious Diseases Society of America, Best abstract at 2004 annual meeting, Boston.
James McDougall Faculty Mentor Award, Fred Hutchinson Cancer Research Center
2007-2013, Associate Professor, University of Washington, School of Medicine, Allergy, and Infectious Diseases
2007-2012, Associate Member, Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division & Clinical Research Division
2001-2007, Assistant Member, Fred Hutchinson Cancer Research Center, Clinical Research Division, Program in Infectious Diseases
2001-2007, Assistant Professor, University of Washington, School of Medicine, Medicine, Allergy and Infectious Diseases
1998-2001, Research Associate, Stanford University, School of Medicine, Medicine, Infectious Diseases
Voriconazole therapeutic drug monitoring: retrospective cohort study of the relationship to clinical outcomes and adverse events.. BMC infectious diseases. 13(1):105.. 2013.
Associations between vaginal bacteria and levels of vaginal defensins in pregnant women.. American journal of obstetrics and gynecology. 208(2):132e.1-7.. 2013.
Extravaginal reservoirs of vaginal bacteria as risk factors for incident bacterial vaginosis.. The Journal of infectious diseases. 205(10):1580-8.. 2012.
Effect of sexual debut on vaginal microbiota in a cohort of young women.. Obstetrics and gynecology. 120(6):1306-13.. 2012.
Enhanced fungal DNA-extraction from formalin-fixed, paraffin-embedded tissue specimens by application of thermal energy.. Medical mycology : official publication of the International Society for Human and Animal Mycology. 50(6):667-72.. 2012.
Behavioral Predictors of Colonization with Lactobacillus crispatus or Lactobacillus jensenii after Treatment for Bacterial Vaginosis: A Cohort Study.. Infectious diseases in obstetrics and gynecology. 2012:706540.. 2012.
Effects of Bacterial Vaginosis-Associated Bacteria and Sexual Intercourse on Vaginal Colonization With the Probiotic Lactobacillus crispatus CTV-05.. Sexually transmitted diseases. 38(11):1020-1027.. 2011.
Randomized, placebo-controlled phase 2 trial of a Lactobacillus crispatus probiotic given intravaginally for prevention of recurrent urinary tract infection.. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 52(10):1212-7.. 2011.
Detection of adeno-associated virus viremia in hematopoietic cell transplant recipients.. The Journal of infectious diseases. 204(11):1746-9.. 2011.
Molecular methods to describe the spectrum and dynamics of the vaginal microbiota.. Anaerobe. 17(4):191-5.. 2011.
Bacterial vaginosis: identifying research gaps proceedings of a workshop sponsored by DHHS/NIH/NIAID.. Sexually transmitted diseases. 37(12):732-44.. 2010.
The impact of a change in antibacterial prophylaxis from ceftazidime to levofloxacin in allogeneic hematopoietic cell transplantation.. Bone marrow transplantation. 45(4):675-81.. 2010.
Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis.. PloS one. 5(4):e10197.. 2010.
Changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative PCR.. Journal of clinical microbiology. 47(3):721-6.. 2009.
Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assays.. Applied and environmental microbiology. 75(6):1559-65.. 2009.
PCR-based diagnosis of human fungal infections.. Expert review of anti-infective therapy. 7(10):1201-21.. 2009.
Comparison of oral and vaginal metronidazole for treatment of bacterial vaginosis in pregnancy: impact on fastidious bacteria.. BMC infectious diseases. 9:89.. 2009.
Detection of fastidious vaginal bacteria in women with HIV infection and bacterial vaginosis.. Infectious diseases in obstetrics and gynecology. 2009:236919.. 2009.
Relationship of specific vaginal bacteria and bacterial vaginosis treatment failure in women who have sex with women.. Annals of internal medicine. 149(1):20-8.. 2008.