Keith R. Jerome
M.D., Duke University, 1993.
Ph.D., Duke University, Immunology, 1992.
B.S., Georgetown College, Chemistry, 1985.
My research focuses on chronic and latent viruses, their immune evasion mechanisms, and potential curative therapeutic approaches to these infections. Recent work involves the use of DNA editing enzymes such as homing endonucleases and zinc finger nucleases to specifically target latent viral DNA for cleavage and inactivation. This approach may allow precise elimination of functional viral DNA from infected cell reservoirs, offering the prospect of cure for HIV, hepatitis B, herpes simplex, and human papillomavirus infections.
Clinically, I serve as Director of the University of Washington molecular virology laboratory, and consult regarding all aspects of virology testing. Specific areas of interest include real time PCR, automation of nucleic acid extraction, and diagnosis of disease caused by herpesviruses, enterovirus, JC and BK viruses, parvovirus B19, and hepatitis virus.
American Society for Microbiology
Infectious Diseases Society of America
Impact of rapid influenza PCR testing on hospitalization and antiviral use: A retrospective cohort study.. Journal of medical virology.. 2015.
Inherited chromosomally integrated human herpesvirus 6 as a predisposing risk factor for the development of angina pectoris.. Proceedings of the National Academy of Sciences of the United States of America.. 2015.
Valganciclovir for the prevention of complications of late cytomegalovirus infection after allogeneic hematopoietic cell transplantation: a randomized trial.. Annals of internal medicine. 162(1):1-10.. 2015.
Comparison of the Simplexa HSV1 & 2 Direct kit and laboratory-developed real-time PCR assays for herpes simplex virus detection.. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 62:103-5.. 2015.
A patient self-collection method for longitudinal monitoring of respiratory virus infection in solid organ transplant recipients.. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 62:98-102.. 2015.
Evaluation of the Clinical Utility of Droplet Digital PCR for Human Cytomegalovirus.. Journal of clinical microbiology.. 2014.
The potential advantages of digital PCR for clinical virology diagnostics.. Expert review of molecular diagnostics. 14(4):501-7.. 2014.
Human herpesvirus 6 can be detected in cerebrospinal fluid without associated symptoms after allogeneic hematopoietic cell transplantation.. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.. 2014.
Past, present, and future perspectives on the diagnosis of Roseolovirus infections.. Current opinion in virology. 9:84-90.. 2014.
In vitro Inactivation of Latent HSV by Targeted Mutagenesis Using an HSV-specific Homing Endonuclease.. Molecular therapy. Nucleic acids. 3:e146.. 2014.
Respiratory Syncytial Virus Lower Respiratory Disease in Hematopoietic Cell Transplant Recipients: Viral RNA Detection in Blood, Antiviral Treatment, and Clinical Outcomes.. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.. 2013.
Robust suppression of env-SHIV viremia in Macaca nemestrina by 3-drug ART is independent of timing of initiation during chronic infection.. Journal of medical primatology. 42(5):237-46.. 2013.
TALENs targeting HBV: designer endonuclease therapies for viral infections.. Molecular therapy : the journal of the American Society of Gene Therapy. 21(10):1819-20.. 2013.
Comparison of the Simplexa FluA/B & RSV Direct Assay and Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Virus.. Journal of clinical microbiology. 51(11):3883-5.. 2013.
Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances.. Clinical chemistry. 59(11):1670-2.. 2013.
Targeted gene disruption to cure HIV.. Current opinion in HIV and AIDS. 8(3):217-23.. 2013.
Investigation of Epstein-Barr Virus as a Potential Cause of B-Cell Non-Hodgkin Lymphoma in a Prospective Cohort.. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.. 2013.
Predictors of hepatitis B cure using gene therapy to deliver DNA cleavage enzymes: a mathematical modeling approach.. PLoS computational biology. 9(7):e1003131.. 2013.
Quantitation of cytomegalovirus DNA load in dried blood spots correlates well with plasma viral load.. Journal of clinical microbiology. 51(7):2360-4.. 2013.