Keith R. Jerome

Appointments and Affiliations
 
 

Fred Hutchinson Cancer Research Center
Associate Member, Vaccine and Infectious Disease Division
Associate Member, Clinical Research Division

University of Washington
Associate Professor, Laboratory Medicine
Adjunct Associate Professor, Microbiology
Head, Virology Division
Director, Molecular Virology Laboratory, Laboratory Medicine

 Professional Headshot of Keith R. Jerome

Mailing Address

1100 Fairview Ave. N.
E5-110
Seattle, Washington 98109
United States

Contact

Phone: (206) 667-6793
kjerome@fhcrc.org

Degrees

M.D., Duke University, 1993.
Ph.D., Duke University, Immunology, 1992.
B.S., Georgetown College, Chemistry, 1985.

Research Interests

My research focuses on chronic and latent viruses, their immune evasion mechanisms, and potential curative therapeutic approaches to these infections. Recent work involves the use of DNA editing enzymes such as homing endonucleases and zinc finger nucleases to specifically target latent viral DNA for cleavage and inactivation. This approach may allow precise elimination of functional viral DNA from infected cell reservoirs, offering the prospect of cure for HIV, hepatitis B, herpes simplex, and human papillomavirus infections.

Clinically, I serve as Director of the University of Washington molecular virology laboratory, and consult regarding all aspects of virology testing. Specific areas of interest include real time PCR, automation of nucleic acid extraction, and diagnosis of disease caused by herpesviruses, enterovirus, JC and BK viruses, parvovirus B19, and hepatitis virus.

Memberships

American Society for Microbiology
Infectious Diseases Society of America

Funding

  • University of Washington Center for AIDS Research (CFAR):  Scientific Working Group – Curative Therapies for HIV, 2013 to 2018.
  • National Institutes of Health (NIH): Target Modification of Host and Proviral DNA to Treat Latent HIV Infection, 2011 to 2015.
  • University of Washington Center for AIDS Research: Dendritic Cell-Based Delivery for Genetic Therapy of Latent HIV Infection,  2011 to 2012.
  • National Institutes of Health (NIH): Hepatitis B Research Network Central Lab, 2010 to 2015.
  • Bill & Melinda Gates Foundation: Homing Endonucleases for the Cure of Latent HIV Infection, 2010 to 2012.
  • Northwest Genome Engineering Consortium/Seattle Children's: Homing Endonucleases for the Cure of Latent HIV Infections in Humans, 2009 to 2011.

 

Recent Publications

2013
Stone, D, Kiem H-P, Jerome KR.  2013.  Targeted gene disruption to cure HIV.. Current opinion in HIV and AIDS. 8(3):217-23. Abstract
Munro, SB, Kuypers J, Jerome KR.  2013.  Comparison of a Multiplex Real-Time PCR Assay with a Multiplex Luminex Assay for Influenza Virus Detection.. Journal of clinical microbiology. Abstract
Xu, M, Qin X, Astion ML, Rutledge JC, Simpson J, Jerome KR, Englund JA, Zerr DM, Migita RT, Rich S et al..  2013.  Implementation of filmarray respiratory viral panel in a core laboratory improves testing turnaround time and patient care.. American journal of clinical pathology. 139(1):118-23. Abstract
Wagner, TA, Lin C-H, Tobin NH, Côté HCF, Sloan DD, Jerome KR, Frenkel LM.  2013.  Quantification of mitochondrial toxicity in HIV-infected individuals by quantitative PCR compared to flow cytometry.. Cytometry. Part B, Clinical cytometry. 84B(1):55-58. Abstract
Sedlak, RH, Jerome KR.  2013.  Viral diagnostics in the era of digital polymerase chain reaction.. Diagnostic microbiology and infectious disease. 75(1):1-4. Abstract
Day, SL, Odem-Davis K, Mandaliya KN, Jerome KR, Cook L, Masese LN, Scott J, Kim HN, Graham SM, McClelland SR.  2013.  Prevalence, clinical and virologic outcomes of hepatitis B virus co-infection in HIV-1 positive Kenyan women on antiretroviral therapy.. PloS one. 8(3):e59346. Abstract
Schiffer, JT, Swan D, Al Sallaq R, Magaret A, Johnston C, Mark KE, Selke S, Ocbamichael N, Kuntz S, Zhu J et al..  2013.  Rapid localized spread and immunologic containment define Herpes simplex virus-2 reactivation in the human genital tract.. eLife. 2:e00288. Abstract
2012
Schiffer, JT, Aubert M, Weber N, Mintzer E, Stone D, Jerome KR.  2012.  Targeted DNA mutagenesis for the cure of chronic viral infections.. Journal of virology. 86(17):8920-36. Abstract
Kiem, H-P, Jerome KR, Deeks SG, McCune JM.  2012.  Hematopoietic-stem-cell-based gene therapy for HIV disease.. Cell stem cell. 10(2):137-47. Abstract
Cook, L, Diem K, Kim W, Scott JD, Jerome KR.  2012.  Allele-Specific PCR for Determination of IL28B Genotype.. Journal of clinical microbiology. 50(12):4144-6. Abstract
Renaud, C, Crowley J, Jerome KR, Kuypers J.  2012.  Comparison of FilmArray Respiratory Panel and laboratory-developed real-time reverse transcription-polymerase chain reaction assays for respiratory virus detection.. Diagnostic microbiology and infectious disease. 74(4):379-83. Abstract
2011
Jerome, KR, Diem K, Huang M-L, Selke S, Corey L, Buchwald D.  2011.  Xenotropic murine leukemia virus-related virus in monozygotic twins discordant for chronic fatigue syndrome.. Diagnostic microbiology and infectious disease. 71(1):66-71. Abstract
Kim, HN, Scott J, Cent A, Cook L, Morrow RA, Richardson BA, Tapia K, Jerome KR, Lule G, John-Stewart G et al..  2011.  HBV lamivudine resistance among hepatitis B and HIV coinfected patients starting lamivudine, stavudine and nevirapine in Kenya.. Journal of viral hepatitis. 18(10):e447-52. Abstract
Wang, CC, Cook L, Tapia KA, Holte S, Krows M, Bagabag A, Santos A, Corey L, Jerome KR.  2011.  Cervicovaginal shedding of hepatitis C viral RNA is associated with the presence of menstrual or other blood in cervicovaginal fluids.. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 50(1):4-7. Abstract
Aubert, M, Ryu BY, Banks L, Rawlings DJ, Scharenberg AM, Jerome KR.  2011.  Successful targeting and disruption of an integrated reporter lentivirus using the engineered homing endonuclease Y2 I-AniI.. PloS one. 6(2):e16825. Abstract
2010
Morishima, C, Shuhart MC, Wang CC, Paschal DM, Apodaca MC, Liu Y, Sloan DD, Graf TN, Oberlies NH, Lee DY-W et al..  2010.  Silymarin inhibits in vitro T-cell proliferation and cytokine production in hepatitis C virus infection.. Gastroenterology. 138(2):671-81,681.e1-2. Abstract
2009
Berrington, WR, Jerome KR, Cook L, Wald A, Corey L, Casper C.  2009.  Clinical correlates of herpes simplex virus viremia among hospitalized adults.. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 49(9):1295-301. Abstract
Lin, C-H, Sloan DD, Dang CH, Wagner T, Cabrera AJE, Tobin NH, Frenkel LM, Jerome KR.  2009.  Assessment of mitochondrial toxicity by analysis of mitochondrial protein expression in mononuclear cells.. Cytometry. Part B, Clinical cytometry. 76(3):181-90. Abstract
Cook, L, Atienza EE, Bagabag A, Obrigewitch RM, Jerome KR.  2009.  Comparison of methods for extraction of viral DNA from cellular specimens.. Diagnostic microbiology and infectious disease. 64(1):37-42. Abstract
Han, J-Y, Miller SA, Wolfe TM, Pourhassan H, Jerome KR.  2009.  Cell type-specific induction and inhibition of apoptosis by Herpes Simplex virus type 2 ICP10.. Journal of virology. 83(6):2765-9. Abstract
Aubert, M, Yoon M, Sloan DD, Spear PG, Jerome KR.  2009.  The virological synapse facilitates herpes simplex virus entry into T cells.. Journal of virology. 83(12):6171-83. Abstract
2008
Jerome, KR.  2008.  Viral modulation of T-cell receptor signaling.. Journal of virology. 82(9):4194-204.
Zahariadis, G, Wagner MJ, Doepker RC, Maciejko JM, Crider CM, Jerome KR, Smiley JR.  2008.  Cell-type-specific tyrosine phosphorylation of the herpes simplex virus tegument protein VP11/12 encoded by gene UL46.. Journal of virology. 82(13):6098-108. Abstract
Aubert, M, Chen Z, Lang R, Dang CH, Fowler C, Sloan DD, Jerome KR.  2008.  The antiapoptotic herpes simplex virus glycoprotein J localizes to multiple cellular organelles and induces reactive oxygen species formation.. Journal of virology. 82(2):617-29. Abstract
Hoffman, NG, Cook L, Atienza EE, Limaye AP, Jerome KR.  2008.  Marked variability of BK virus load measurement using quantitative real-time PCR among commonly used assays.. Journal of clinical microbiology. 46(8):2671-80. Abstract