Keith R. Jerome
M.D., Duke University, 1993.
Ph.D., Duke University, Immunology, 1992.
B.S., Georgetown College, Chemistry, 1985.
My research focuses on chronic and latent viruses, their immune evasion mechanisms, and potential curative therapeutic approaches to these infections. Recent work involves the use of DNA editing enzymes such as homing endonucleases and zinc finger nucleases to specifically target latent viral DNA for cleavage and inactivation. This approach may allow precise elimination of functional viral DNA from infected cell reservoirs, offering the prospect of cure for HIV, hepatitis B, herpes simplex, and human papillomavirus infections.
Clinically, I serve as Director of the University of Washington molecular virology laboratory, and consult regarding all aspects of virology testing. Specific areas of interest include real time PCR, automation of nucleic acid extraction, and diagnosis of disease caused by herpesviruses, enterovirus, JC and BK viruses, parvovirus B19, and hepatitis virus.
American Society for Microbiology
Infectious Diseases Society of America
Evaluation of the Clinical Utility of Droplet Digital PCR for Human Cytomegalovirus.. Journal of clinical microbiology.. 2014.
The potential advantages of digital PCR for clinical virology diagnostics.. Expert review of molecular diagnostics. 14(4):501-7.. 2014.
Human herpesvirus 6 can be detected in cerebrospinal fluid without associated symptoms after allogeneic hematopoietic cell transplantation.. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.. 2014.
In vitro Inactivation of Latent HSV by Targeted Mutagenesis Using an HSV-specific Homing Endonuclease.. Molecular therapy. Nucleic acids. 3:e146.. 2014.
Respiratory Syncytial Virus Lower Respiratory Disease in Hematopoietic Cell Transplant Recipients: Viral RNA Detection in Blood, Antiviral Treatment, and Clinical Outcomes.. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.. 2013.
Robust suppression of env-SHIV viremia in Macaca nemestrina by 3-drug ART is independent of timing of initiation during chronic infection.. Journal of medical primatology. 42(5):237-46.. 2013.
TALENs targeting HBV: designer endonuclease therapies for viral infections.. Molecular therapy : the journal of the American Society of Gene Therapy. 21(10):1819-20.. 2013.
Comparison of the Simplexa FluA/B & RSV Direct Assay and Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Virus.. Journal of clinical microbiology. 51(11):3883-5.. 2013.
Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances.. Clinical chemistry. 59(11):1670-2.. 2013.
Targeted gene disruption to cure HIV.. Current opinion in HIV and AIDS. 8(3):217-23.. 2013.
Investigation of Epstein-Barr Virus as a Potential Cause of B-Cell Non-Hodgkin Lymphoma in a Prospective Cohort.. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.. 2013.
Predictors of hepatitis B cure using gene therapy to deliver DNA cleavage enzymes: a mathematical modeling approach.. PLoS computational biology. 9(7):e1003131.. 2013.
Quantitation of cytomegalovirus DNA load in dried blood spots correlates well with plasma viral load.. Journal of clinical microbiology. 51(7):2360-4.. 2013.
Rapid Detection of Human Cytomegalovirus UL97 and UL54 Mutations Directly from Patient Samples.. Journal of clinical microbiology. 51(7):2354-9.. 2013.
Comparison of a Multiplex Real-Time PCR Assay with a Multiplex Luminex Assay for Influenza Virus Detection.. Journal of clinical microbiology.. 2013.
Implementation of filmarray respiratory viral panel in a core laboratory improves testing turnaround time and patient care.. American journal of clinical pathology. 139(1):118-23.. 2013.
Quantification of mitochondrial toxicity in HIV-infected individuals by quantitative PCR compared to flow cytometry.. Cytometry. Part B, Clinical cytometry. 84B(1):55-58.. 2013.
Viral diagnostics in the era of digital polymerase chain reaction.. Diagnostic microbiology and infectious disease. 75(1):1-4.. 2013.
Targeted DNA mutagenesis for the cure of chronic viral infections.. Journal of virology. 86(17):8920-36.. 2012.
Hematopoietic-stem-cell-based gene therapy for HIV disease.. Cell stem cell. 10(2):137-47.. 2012.