Monoclonal Antibody Development

Location: 1100 Eastlake Building, E1-140
Contact phone: (206) 667-6612
Contact e-mail: bhoffstr@fhcrc.org

Our Services Include:

  • Design of antigens, immunization and screening strategies. This includes peptide design and decisions regarding the selection of multiple antigenic or branched peptides (MAPs) versus KLH (or other carrier protein)-conjugates with linear peptides, antigen purification and solubilization, and the optimization of screening protocols.
  • Hybridoma cell line production via traditional methods. This includes quality control of protein or peptide immunogen samples, initial screening of polyclonal antiserum via ELISA, Western blot, and immune precipitation, fusion, post-fusion screening, positive well expansion and subcloning of hybridomas secreting monoclonal antibodies with the desired specificity
  • Conjugation of linear peptides to carrier molecules such as KLH or BSA. This includes quality control of peptide conjugates via SDS-PAGE gel analysis and mass spectrometry.
  • Production of monoclonal antibodies in species other than mice. Currently, we have rat and rabbit myeloma cell lines. The resulting antibody secreting cell lines are useful in situations where investigators need monoclonal antibodies directed towards murine antigens, or when human antigens are not immunogenic in mice.
  • The facility also provides monoclonal antibodies directed to a number of biological tags and reporter molecules (See Products). These include green fluorescent protein, glutathione S transferase, maltose binding protein, myc, 6-H, M13 phage (native or denatured), wheat germ agglutinin, and other antibodies directed to members of the integrin and Ig-CAM families.

Since each hybridoma development project is unique and successful projects will often require that immunization and screening protocols be tailored to the unique needs of the investigator, we have the expertise available to assist investigators to design the exact screening mechanism to produce the desired reagent.

Contact resource staff for initial consultation and project scheduling (206) 667-6612.

Doing Business with Antibody Development

1100 Eastlake Building, E1-140
(206) 667-6612
bhoffstr@fhcrc.org

More About Antibody Development